The progress of intestinal epithelial models from cell lines to gut-on-chip

Shafaque Rahman, Mohammed Ghiboub, Joanne M. Donkers, Evita van de Steeg, Eric A. F. van Tol, Theodorus B. M. Hakvoort, Wouter J. de Jonge

Research output: Contribution to journalReview articleAcademicpeer-review

30 Citations (Scopus)

Abstract

Over the past years, several preclinical in vitro and ex vivo models have been developed that helped to understand some of the critical aspects of intestinal functions in health and disease such as inflammatory bowel disease (IBD). However, the translation to the human in vivo situation remains problematic. The main reason for this is that these approaches fail to fully reflect the multifactorial and complex in vivo environment (e.g., including microbiota, nutrition, and immune response) in the gut system. Although conventional models such as cell lines, Ussing chamber, and the everted sac are still used, increasingly more sophisticated intestinal models have been developed over the past years including organoids, InTESTine™ and microfluidic gut-on-chip. In this review, we gathered the most recent insights on the setup, advantages, limitations, and future perspectives of most frequently used in vitro and ex vivo models to study intestinal physiology and functions in health and disease.
Original languageEnglish
Article number13472
JournalInternational journal of molecular sciences
Volume22
Issue number24
DOIs
Publication statusPublished - 1 Dec 2021

Keywords

  • Ex vivo
  • Gut-on-chip
  • In vitro
  • Inflammation
  • Intestine
  • Organoids

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