TY - JOUR
T1 - The transcription factor Spi-B is expressed in plasmacytoid DC precursors and inhibits T-, B-, and NK-cell development
AU - Schotte, Remko
AU - Rissoan, Marie-Clotilde
AU - Bendriss-Vermare, Nathalie
AU - Bridon, Jean-Michel
AU - Duhen, Thomas
AU - Weijer, Kees
AU - Brière, Francine
AU - Spits, Hergen
PY - 2003
Y1 - 2003
N2 - Human plasmacytoid dendritic cells (pCs), also called type 2 dendritic cell precursors or natural interferon (IFN)producing cells, represent a cell type with distinctive phenotypic and functional features. They are present in the thymus and probably share a common precursor with T and natural killer (INK) cells. In an effort to identify gene's that control pDC development we searched for genes of which the expression is restricted to human pDC using a cDNA subtraction technique with activated monocyte-derived DCs (MoDCs) as competitor. We identified the transcription factor Spi-B to be expressed in pDCs but not in Mo-DCs. Spi-B expression in pDCs was maintained on in vitro maturation of pDCs. Spi-B was expressed in early CD34(+)CD38(-) hematopoietic progenitors and in CD34(+)CD1a(-) thymic precursors. Spi-B expression is down-regulated when uncommitted CD34(+)CD1a(-) thymic precursors differentiate into committed CD34(+)CD1a(+) pre-T cells. Overexpression of Spi-B in hematopoietic progenitor cells resulted in inhibition of development of T cells both in vitro and in vivo. In addition, development of progenitor cells into B and INK cells in vitro was also inhibited by Spi-B overexpression. Our results indicate that Spi-B is involved in the control of pDC development by limiting the capacity of progenitor cells to develop into other lymphoid lineages. (C) 2003 by The American Society of Hematology
AB - Human plasmacytoid dendritic cells (pCs), also called type 2 dendritic cell precursors or natural interferon (IFN)producing cells, represent a cell type with distinctive phenotypic and functional features. They are present in the thymus and probably share a common precursor with T and natural killer (INK) cells. In an effort to identify gene's that control pDC development we searched for genes of which the expression is restricted to human pDC using a cDNA subtraction technique with activated monocyte-derived DCs (MoDCs) as competitor. We identified the transcription factor Spi-B to be expressed in pDCs but not in Mo-DCs. Spi-B expression in pDCs was maintained on in vitro maturation of pDCs. Spi-B was expressed in early CD34(+)CD38(-) hematopoietic progenitors and in CD34(+)CD1a(-) thymic precursors. Spi-B expression is down-regulated when uncommitted CD34(+)CD1a(-) thymic precursors differentiate into committed CD34(+)CD1a(+) pre-T cells. Overexpression of Spi-B in hematopoietic progenitor cells resulted in inhibition of development of T cells both in vitro and in vivo. In addition, development of progenitor cells into B and INK cells in vitro was also inhibited by Spi-B overexpression. Our results indicate that Spi-B is involved in the control of pDC development by limiting the capacity of progenitor cells to develop into other lymphoid lineages. (C) 2003 by The American Society of Hematology
U2 - https://doi.org/10.1182/blood-2002-02-0438
DO - https://doi.org/10.1182/blood-2002-02-0438
M3 - Article
C2 - 12393575
SN - 0006-4971
VL - 101
SP - 1015
EP - 1023
JO - Blood
JF - Blood
IS - 3
ER -