TY - JOUR
T1 - Transient hypothyroxinemia in juvenile glycoprotein hormone subunit B5 knock-out mice
AU - van Zeijl, Clementine J. J.
AU - Surovtseva, Olga V.
AU - Wiersinga, Wilmar M.
AU - Boelen, Anita
AU - Fliers, Eric
PY - 2010
Y1 - 2010
N2 - The heterodimer thyrostimulin, comprised of two novel glycoprotein hormone subunits GPA2 and GPB5, activates the TSH receptor. To understand its role in the regulation of the hypothalamus-pituitary-thyroid (HPT-) axis, we evaluated juvenile and adult GPB5 knock-out (GPB5(-/-)) and wild type mice own during euthyroidism, hypothyroidism and thyrotoxicosis Surprisingly, juvenile euthyroid GPB5(-/-) mice displayed marked hypothyroxinemia (25% lower serum T-4, unchanged TSH) and also during thyrotoxicosis juvenile GPB5(-/-) mice had 25% lower serum T-4, compared to WT During hypothyroidism, despite similar serum T-4, pituitary TSH beta mRNA was 2-fold lower in GPB5(-/-) mice compared to WT Adult mice displayed increased pituitary deiodinase type 2 during euthyroidism and decreased serum T-4 during hypothyroidism in GPB5(-/-). Thus, lacking GPB5 results in moderate deviations of the HPT-axis. The more pronounced differences observed in juvenile mice compared to adult mice are in agreement with the notion that GPB5 has a role during development (C) 2010 Elsevier Ireland Ltd All rights reserved
AB - The heterodimer thyrostimulin, comprised of two novel glycoprotein hormone subunits GPA2 and GPB5, activates the TSH receptor. To understand its role in the regulation of the hypothalamus-pituitary-thyroid (HPT-) axis, we evaluated juvenile and adult GPB5 knock-out (GPB5(-/-)) and wild type mice own during euthyroidism, hypothyroidism and thyrotoxicosis Surprisingly, juvenile euthyroid GPB5(-/-) mice displayed marked hypothyroxinemia (25% lower serum T-4, unchanged TSH) and also during thyrotoxicosis juvenile GPB5(-/-) mice had 25% lower serum T-4, compared to WT During hypothyroidism, despite similar serum T-4, pituitary TSH beta mRNA was 2-fold lower in GPB5(-/-) mice compared to WT Adult mice displayed increased pituitary deiodinase type 2 during euthyroidism and decreased serum T-4 during hypothyroidism in GPB5(-/-). Thus, lacking GPB5 results in moderate deviations of the HPT-axis. The more pronounced differences observed in juvenile mice compared to adult mice are in agreement with the notion that GPB5 has a role during development (C) 2010 Elsevier Ireland Ltd All rights reserved
U2 - https://doi.org/10.1016/j.mce.2010.03.002
DO - https://doi.org/10.1016/j.mce.2010.03.002
M3 - Article
C2 - 20223276
SN - 0303-7207
VL - 321
SP - 231
EP - 238
JO - Molecular and cellular endocrinology
JF - Molecular and cellular endocrinology
IS - 2
ER -