TY - JOUR
T1 - Multiple Ca2+ sensors in secretion
T2 - Teammates, competitors or autocrats?
AU - Walter, Alexander M.
AU - Groffen, Alexander J.
AU - Sørensen Jakob B., J. B.
AU - Verhage, Matthijs
AU - Sorensen, J.B.
N1 - Funding Information: We apologize to the authors of many important studies that we could not discuss because of space constraints. We thank Tina Kunit-Marquardt for graphical representations of the models. We also thank Niels Cornelisse, Josep Rizo, Keimpe D.B. Wierda, Paulo Pinheiro and Rocio Diez Arazola for comments on the manuscript. This work was supported by the Lundbeck Foundation [Junior Group Leader Fellowship and the Lundbeck Foundation Center for Biomembranes in Nanomedicine (JBS)], the Danish Medical Research Council (JBS), the European Molecular Biology Organization (EMBO; AMW) and the European Union Seventh Framework Program under grant agreement HEALTH-F2-2009-242167 (‘SynSys’ project; MV and JBS).
PY - 2011/9
Y1 - 2011/9
N2 - Regulated neurotransmitter secretion depends on Ca 2+ sensors, C2 domain proteins that associate with phospholipids and soluble N-ethylmaleimide-sensitive fusion attachment protein receptor (SNARE) complexes to trigger release upon Ca 2+ binding. Ca 2+ sensors are thought to prevent spontaneous fusion at rest (clamping) and to promote fusion upon Ca 2+ activation. At least eight, often coexpressed, Ca 2+ sensors have been identified in mammals. Accumulating evidence suggests that multiple Ca 2+ sensors interact, rather than work autonomously, to produce the complex secretory response observed in neurons and secretory cells. In this review, we present several working models to describe how different sensors might be arranged to mediate synchronous, asynchronous and spontaneous neurotransmitter release. We discuss the scenario that different Ca 2+ sensors typically act on one shared vesicle pool and compete for binding the multiple SNARE complexes that are likely to assemble at single vesicles, to exert both clamping and fusion-promoting functions.
AB - Regulated neurotransmitter secretion depends on Ca 2+ sensors, C2 domain proteins that associate with phospholipids and soluble N-ethylmaleimide-sensitive fusion attachment protein receptor (SNARE) complexes to trigger release upon Ca 2+ binding. Ca 2+ sensors are thought to prevent spontaneous fusion at rest (clamping) and to promote fusion upon Ca 2+ activation. At least eight, often coexpressed, Ca 2+ sensors have been identified in mammals. Accumulating evidence suggests that multiple Ca 2+ sensors interact, rather than work autonomously, to produce the complex secretory response observed in neurons and secretory cells. In this review, we present several working models to describe how different sensors might be arranged to mediate synchronous, asynchronous and spontaneous neurotransmitter release. We discuss the scenario that different Ca 2+ sensors typically act on one shared vesicle pool and compete for binding the multiple SNARE complexes that are likely to assemble at single vesicles, to exert both clamping and fusion-promoting functions.
UR - http://www.scopus.com/inward/record.url?scp=80051933736&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.tins.2011.07.003
DO - https://doi.org/10.1016/j.tins.2011.07.003
M3 - Review article
C2 - 21831459
SN - 0166-2236
VL - 34
SP - 487
EP - 497
JO - Trends in Neurosciences
JF - Trends in Neurosciences
IS - 9
ER -