TY - JOUR
T1 - Non-Polio Enterovirus C Replicate in Both Airway and Intestine Organotypic Cultures
AU - Moreni, Giulia
AU - van Eijk, Hetty
AU - Koen, Gerrit
AU - Johannesson, Nina
AU - Calitz, Carlemi
AU - Benschop, Kimberley
AU - Cremer, Jeroen
AU - Pajkrt, Dasja
AU - Sridhar, Adithya
AU - Wolthers, Katja
N1 - Funding Information: This work was co-funded by the European Union’s Horizon 2020 Research and Innovation Programme under the Marie Sklowdowska-Curie Grant Agreement OrganoVIR (grant 812673), the European Unions’s Horizon 2020 Research and Innovation Programme under the grant agreement GUTVIBRATIONS (grant 953201), and the PPP Allowance (Focus-on-Virus) made available by Health Holland, Top Sector Life Sciences & Health, to the Amsterdam UMC, location Amsterdam Medical Center, to stimulate public–private partnerships. Publisher Copyright: © 2023 by the authors.
PY - 2023/9/1
Y1 - 2023/9/1
N2 - Non-polio enteroviruses (EV) belonging to species C, which are highly prevalent in Africa, mainly among children, are poorly characterized, and their pathogenesis is mostly unknown as they are difficult to culture. In this study, human airway and intestinal organotypic models were used to investigate tissue and cellular tropism of three EV-C genotypes, EV-C99, CVA-13, and CVA-20. Clinical isolates were obtained within the two passages of culture on Caco2 cells, and all three viruses were replicated in both the human airway and intestinal organotypic cultures. We did not observe differences in viral replication between fetal and adult tissue that could potentially explain the preferential infection of infants by EV-C genotypes. Infection of the airway and the intestinal cultures indicates that they both can serve as entry sites for non-polio EV-C. Ciliated airway cells and enterocytes are the target of infection for all three viruses, as well as enteroendocrine cells for EV-C99.
AB - Non-polio enteroviruses (EV) belonging to species C, which are highly prevalent in Africa, mainly among children, are poorly characterized, and their pathogenesis is mostly unknown as they are difficult to culture. In this study, human airway and intestinal organotypic models were used to investigate tissue and cellular tropism of three EV-C genotypes, EV-C99, CVA-13, and CVA-20. Clinical isolates were obtained within the two passages of culture on Caco2 cells, and all three viruses were replicated in both the human airway and intestinal organotypic cultures. We did not observe differences in viral replication between fetal and adult tissue that could potentially explain the preferential infection of infants by EV-C genotypes. Infection of the airway and the intestinal cultures indicates that they both can serve as entry sites for non-polio EV-C. Ciliated airway cells and enterocytes are the target of infection for all three viruses, as well as enteroendocrine cells for EV-C99.
KW - CVA-13
KW - CVA-20
KW - EV-C99
KW - enterovirus C species
KW - human airway epithelium
KW - human intestinal epithelium
KW - non-polio enterovirus
KW - organoids
KW - organotypic cultures
KW - tropism
UR - http://www.scopus.com/inward/record.url?scp=85172420510&partnerID=8YFLogxK
U2 - https://doi.org/10.3390/v15091823
DO - https://doi.org/10.3390/v15091823
M3 - Article
C2 - 37766230
SN - 1999-4915
VL - 15
JO - Viruses
JF - Viruses
IS - 9
M1 - 1823
ER -