Presenting native-like HIV-1 envelope trimers on ferritin nanoparticles improves their immunogenicity

Kwinten Sliepen; Gabriel Ozorowski; Judith A. Burger; Thijs van Montfort; Melissa Stunnenberg; Celia Labranche; David C. Montefiori; John P. Moore; Andrew B. Ward; Rogier W. Sanders

doi:https://doi.org/10.1186/s12977-015-0210-4

Presenting native-like HIV-1 envelope trimers on ferritin nanoparticles improves their immunogenicity

Kwinten Sliepen, Gabriel Ozorowski, Judith A. Burger, Thijs van Montfort, Melissa Stunnenberg, Celia Labranche, David C. Montefiori, John P. Moore, Andrew B. Ward, Rogier W. Sanders

Research output: Contribution to journal › Article › Academic › peer-review

133 Citations (Scopus)

Abstract

Background: Presenting vaccine antigens in particulate form can improve their immunogenicity by enhancing B cell activation. Findings: We describe ferritin-based protein nanoparticles that display multiple copies of native-like HIV-1 envelope glycoprotein trimers (BG505 SOSIP.664). Trimer-bearing nanoparticles were significantly more immunogenic than trimers in both mice and rabbits. Furthermore, rabbits immunized with the trimer-bearing nanoparticles induced significantly higher neutralizing antibody responses against most tier 1A viruses, and higher responses ( but not significantly), to several tier 1B viruses and the autologous tier 2 virus than when the same trimers were delivered as soluble proteins. Conclusions: This or other nanoparticle designs may be practical ways to improve the immunogenicity of envelope glycoprotein trimers

Original language	English
Pages (from-to)	82
Journal	Retrovirology
Volume	12
Issue number	1
DOIs	https://doi.org/10.1186/s12977-015-0210-4
Publication status	Published - 2015

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https://doi.org/10.1186/s12977-015-0210-4

Cite this

@article{37a6efb4c9d847c4ad88bc5b64224dd8,

title = "Presenting native-like HIV-1 envelope trimers on ferritin nanoparticles improves their immunogenicity",

abstract = "Background: Presenting vaccine antigens in particulate form can improve their immunogenicity by enhancing B cell activation. Findings: We describe ferritin-based protein nanoparticles that display multiple copies of native-like HIV-1 envelope glycoprotein trimers (BG505 SOSIP.664). Trimer-bearing nanoparticles were significantly more immunogenic than trimers in both mice and rabbits. Furthermore, rabbits immunized with the trimer-bearing nanoparticles induced significantly higher neutralizing antibody responses against most tier 1A viruses, and higher responses ( but not significantly), to several tier 1B viruses and the autologous tier 2 virus than when the same trimers were delivered as soluble proteins. Conclusions: This or other nanoparticle designs may be practical ways to improve the immunogenicity of envelope glycoprotein trimers",

author = "Kwinten Sliepen and Gabriel Ozorowski and Burger, {Judith A.} and {van Montfort}, Thijs and Melissa Stunnenberg and Celia Labranche and Montefiori, {David C.} and Moore, {John P.} and Ward, {Andrew B.} and Sanders, {Rogier W.}",

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T1 - Presenting native-like HIV-1 envelope trimers on ferritin nanoparticles improves their immunogenicity

AU - Sliepen, Kwinten

AU - Ozorowski, Gabriel

AU - Burger, Judith A.

AU - van Montfort, Thijs

AU - Stunnenberg, Melissa

AU - Labranche, Celia

AU - Montefiori, David C.

AU - Moore, John P.

AU - Ward, Andrew B.

AU - Sanders, Rogier W.

PY - 2015

Y1 - 2015

N2 - Background: Presenting vaccine antigens in particulate form can improve their immunogenicity by enhancing B cell activation. Findings: We describe ferritin-based protein nanoparticles that display multiple copies of native-like HIV-1 envelope glycoprotein trimers (BG505 SOSIP.664). Trimer-bearing nanoparticles were significantly more immunogenic than trimers in both mice and rabbits. Furthermore, rabbits immunized with the trimer-bearing nanoparticles induced significantly higher neutralizing antibody responses against most tier 1A viruses, and higher responses ( but not significantly), to several tier 1B viruses and the autologous tier 2 virus than when the same trimers were delivered as soluble proteins. Conclusions: This or other nanoparticle designs may be practical ways to improve the immunogenicity of envelope glycoprotein trimers

AB - Background: Presenting vaccine antigens in particulate form can improve their immunogenicity by enhancing B cell activation. Findings: We describe ferritin-based protein nanoparticles that display multiple copies of native-like HIV-1 envelope glycoprotein trimers (BG505 SOSIP.664). Trimer-bearing nanoparticles were significantly more immunogenic than trimers in both mice and rabbits. Furthermore, rabbits immunized with the trimer-bearing nanoparticles induced significantly higher neutralizing antibody responses against most tier 1A viruses, and higher responses ( but not significantly), to several tier 1B viruses and the autologous tier 2 virus than when the same trimers were delivered as soluble proteins. Conclusions: This or other nanoparticle designs may be practical ways to improve the immunogenicity of envelope glycoprotein trimers

U2 - https://doi.org/10.1186/s12977-015-0210-4

DO - https://doi.org/10.1186/s12977-015-0210-4

M3 - Article

C2 - 26410741

SN - 1742-4690

VL - 12

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JO - Retrovirology

JF - Retrovirology

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