TY - JOUR
T1 - Sheltering behavior and locomotor activity in 11 genetically diverse common inbred mouse strains using home-cage monitoring
AU - Aarts, E.
AU - Maroteaux, G.P.
AU - van der Sluis, S.
AU - Verhage, M.
AU - Smit, A.B.
AU - Loos, M.
AU - Koopmans, B.
AU - Brussaard, A.B.
AU - Borst, J.G.
AU - Elgersma, Y
AU - Galjart, N.
AU - van der Horst, G.T.
AU - Levelt, C.N.
AU - Pennartz, C.M.
AU - Spruit, B.M.
AU - de Zeeuw, C.I.
N1 - With The Neuro-BSIK Mouse Phenomics consortium
PY - 2014
Y1 - 2014
N2 - Functional genetic analyses in mice rely on efficient and in-depth characterization of the behavioral spectrum. Automated home-cage observation can provide a systematic and efficient screening method to detect unexplored, novel behavioral phenotypes. Here, we analyzed high-throughput automated home-cage data using existing and novel concepts, to detect a plethora of genetic differences in spontaneous behavior in a panel of commonly used inbred strains (129S1/SvImJ, A/J, C3H/HeJ, C57BL/6J, BALB/cJ, DBA/2J, NOD/LtJ, FVB/NJ, WSB/EiJ, PWK/PhJ and CAST/EiJ). Continuous video-tracking observations of sheltering behavior and locomotor activity were segmented into distinguishable behavioral elements, and studied at different time scales, yielding a set of 115 behavioral parameters of which 105 showed highly significant strain differences. This set of 115 parameters was highly dimensional; principal component analysis identified 26 orthogonal components with eigenvalues above one. Especially novel parameters of sheltering behavior and parameters describing aspects of motion of the mouse in the home-cage showed high genetic effect sizes. Multi-day habituation curves and patterns of behavior surrounding dark/light phase transitions showed striking strain differences, albeit with lower genetic effect sizes. This spontaneous home-cage behavior study demonstrates high dimensionality, with a strong genetic contribution to specific sets of behavioral measures. Importantly, spontaneous home-cage behavior analysis detects genetic effects that cannot be studied in conventional behavioral tests, showing that the inclusion of a few days of undisturbed, labor extensive homecage assessment may greatly aid gene function analyses and drug target discovery.
AB - Functional genetic analyses in mice rely on efficient and in-depth characterization of the behavioral spectrum. Automated home-cage observation can provide a systematic and efficient screening method to detect unexplored, novel behavioral phenotypes. Here, we analyzed high-throughput automated home-cage data using existing and novel concepts, to detect a plethora of genetic differences in spontaneous behavior in a panel of commonly used inbred strains (129S1/SvImJ, A/J, C3H/HeJ, C57BL/6J, BALB/cJ, DBA/2J, NOD/LtJ, FVB/NJ, WSB/EiJ, PWK/PhJ and CAST/EiJ). Continuous video-tracking observations of sheltering behavior and locomotor activity were segmented into distinguishable behavioral elements, and studied at different time scales, yielding a set of 115 behavioral parameters of which 105 showed highly significant strain differences. This set of 115 parameters was highly dimensional; principal component analysis identified 26 orthogonal components with eigenvalues above one. Especially novel parameters of sheltering behavior and parameters describing aspects of motion of the mouse in the home-cage showed high genetic effect sizes. Multi-day habituation curves and patterns of behavior surrounding dark/light phase transitions showed striking strain differences, albeit with lower genetic effect sizes. This spontaneous home-cage behavior study demonstrates high dimensionality, with a strong genetic contribution to specific sets of behavioral measures. Importantly, spontaneous home-cage behavior analysis detects genetic effects that cannot be studied in conventional behavioral tests, showing that the inclusion of a few days of undisturbed, labor extensive homecage assessment may greatly aid gene function analyses and drug target discovery.
UR - https://pure.uva.nl/ws/files/2347781/162588_Sheltering_behavior_and_locomotor_activity_suppl._1.pdf
UR - https://pure.uva.nl/ws/files/2347783/162589_Sheltering_behavior_and_locomotor_activity_suppl._2.pdf
UR - https://pure.uva.nl/ws/files/2347785/162590_Sheltering_behavior_and_locomotor_activity_suppl._3.pdf
UR - https://pure.uva.nl/ws/files/2347787/162591_Sheltering_behavior_and_locomotor_activity_suppl._4.pdf
U2 - https://doi.org/10.1371/journal.pone.0108563
DO - https://doi.org/10.1371/journal.pone.0108563
M3 - Article
C2 - 25264768
SN - 1932-6203
VL - 9
SP - e108563
JO - PLOS ONE
JF - PLOS ONE
IS - 9
M1 - e108563
ER -
