The Adhesion G Protein-Coupled Receptor GPR56/ADGRG1 Is an Inhibitory Receptor on Human NK Cells

Gin-Wen Chang; Cheng-Chih Hsiao; Yen-Ming Peng; Felipe A. Vieira Braga; Natasja A. M. Kragten; Ester B. M. Remmerswaal; Martijn D. B. van de Garde; Rachel Straussberg; Gabriele M. König; Evi Kostenis; Vera Knäuper; Linde Meyaard; René A. W. van Lier; Klaas P. J. M. van Gisbergen; Hsi-Hsien Lin; Jörg Hamann

doi:https://doi.org/10.1016/j.celrep.2016.04.053

The Adhesion G Protein-Coupled Receptor GPR56/ADGRG1 Is an Inhibitory Receptor on Human NK Cells

Gin-Wen Chang, Cheng-Chih Hsiao, Yen-Ming Peng, Felipe A. Vieira Braga, Natasja A. M. Kragten, Ester B. M. Remmerswaal, Martijn D. B. van de Garde, Rachel Straussberg, Gabriele M. König, Evi Kostenis, Vera Knäuper, Linde Meyaard, René A. W. van Lier, Klaas P. J. M. van Gisbergen, Hsi-Hsien Lin, Jörg Hamann

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Natural killer (NK) cells possess potent cytotoxic mechanisms that need to be tightly controlled. Here, we explored the regulation and function of GPR56/ADGRG1, an adhesion G protein-coupled receptor implicated in developmental processes and expressed distinctively in mature NK cells. Expression of GPR56 was triggered by Hobit (a homolog of Blimp-1 in T cells) and declined upon cell activation. Through studying NK cells from polymicrogyria patients with disease-causing mutations in ADGRG1, encoding GPR56, and NK-92 cells ectopically expressing the receptor, we found that GPR56 negatively regulates immediate effector functions, including production of inflammatory cytokines and cytolytic proteins, degranulation, and target cell killing. GPR56 pursues this activity by associating with the tetraspanin CD81. We conclude that GPR56 inhibits natural cytotoxicity of human NK cells

Original language	English
Pages (from-to)	1757-1770
Journal	Cell reports
Volume	15
Issue number	8
DOIs	https://doi.org/10.1016/j.celrep.2016.04.053
Publication status	Published - 2016

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https://doi.org/10.1016/j.celrep.2016.04.053

Cite this

Chang, G.-W., Hsiao, C.-C., Peng, Y.-M., Vieira Braga, F. A., Kragten, N. A. M., Remmerswaal, E. B. M., van de Garde, M. D. B., Straussberg, R., König, G. M., Kostenis, E., Knäuper, V., Meyaard, L., van Lier, R. A. W., van Gisbergen, K. P. J. M., Lin, H.-H., & Hamann, J. (2016). The Adhesion G Protein-Coupled Receptor GPR56/ADGRG1 Is an Inhibitory Receptor on Human NK Cells. Cell reports, 15(8), 1757-1770. https://doi.org/10.1016/j.celrep.2016.04.053

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title = "The Adhesion G Protein-Coupled Receptor GPR56/ADGRG1 Is an Inhibitory Receptor on Human NK Cells",

abstract = "Natural killer (NK) cells possess potent cytotoxic mechanisms that need to be tightly controlled. Here, we explored the regulation and function of GPR56/ADGRG1, an adhesion G protein-coupled receptor implicated in developmental processes and expressed distinctively in mature NK cells. Expression of GPR56 was triggered by Hobit (a homolog of Blimp-1 in T cells) and declined upon cell activation. Through studying NK cells from polymicrogyria patients with disease-causing mutations in ADGRG1, encoding GPR56, and NK-92 cells ectopically expressing the receptor, we found that GPR56 negatively regulates immediate effector functions, including production of inflammatory cytokines and cytolytic proteins, degranulation, and target cell killing. GPR56 pursues this activity by associating with the tetraspanin CD81. We conclude that GPR56 inhibits natural cytotoxicity of human NK cells",

author = "Gin-Wen Chang and Cheng-Chih Hsiao and Yen-Ming Peng and {Vieira Braga}, {Felipe A.} and Kragten, {Natasja A. M.} and Remmerswaal, {Ester B. M.} and {van de Garde}, {Martijn D. B.} and Rachel Straussberg and K{\"o}nig, {Gabriele M.} and Evi Kostenis and Vera Kn{\"a}uper and Linde Meyaard and {van Lier}, {Ren{\'e} A. W.} and {van Gisbergen}, {Klaas P. J. M.} and Hsi-Hsien Lin and J{\"o}rg Hamann",

year = "2016",

doi = "https://doi.org/10.1016/j.celrep.2016.04.053",

language = "English",

volume = "15",

pages = "1757--1770",

journal = "Cell reports",

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Chang, G-W, Hsiao, C-C, Peng, Y-M, Vieira Braga, FA, Kragten, NAM , Remmerswaal, EBM, van de Garde, MDB, Straussberg, R, König, GM, Kostenis, E, Knäuper, V, Meyaard, L, van Lier, RAW , van Gisbergen, KPJM, Lin, H-H & Hamann, J 2016, 'The Adhesion G Protein-Coupled Receptor GPR56/ADGRG1 Is an Inhibitory Receptor on Human NK Cells', Cell reports, vol. 15, no. 8, pp. 1757-1770. https://doi.org/10.1016/j.celrep.2016.04.053

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AU - Chang, Gin-Wen

AU - Hsiao, Cheng-Chih

AU - Peng, Yen-Ming

AU - Vieira Braga, Felipe A.

AU - Kragten, Natasja A. M.

AU - Remmerswaal, Ester B. M.

AU - van de Garde, Martijn D. B.

AU - Straussberg, Rachel

AU - König, Gabriele M.

AU - Kostenis, Evi

AU - Knäuper, Vera

AU - Meyaard, Linde

AU - van Lier, René A. W.

AU - van Gisbergen, Klaas P. J. M.

AU - Lin, Hsi-Hsien

AU - Hamann, Jörg

PY - 2016

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AB - Natural killer (NK) cells possess potent cytotoxic mechanisms that need to be tightly controlled. Here, we explored the regulation and function of GPR56/ADGRG1, an adhesion G protein-coupled receptor implicated in developmental processes and expressed distinctively in mature NK cells. Expression of GPR56 was triggered by Hobit (a homolog of Blimp-1 in T cells) and declined upon cell activation. Through studying NK cells from polymicrogyria patients with disease-causing mutations in ADGRG1, encoding GPR56, and NK-92 cells ectopically expressing the receptor, we found that GPR56 negatively regulates immediate effector functions, including production of inflammatory cytokines and cytolytic proteins, degranulation, and target cell killing. GPR56 pursues this activity by associating with the tetraspanin CD81. We conclude that GPR56 inhibits natural cytotoxicity of human NK cells

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DO - https://doi.org/10.1016/j.celrep.2016.04.053

M3 - Article

C2 - 27184850

SN - 2211-1247

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