TY - JOUR
T1 - Discriminating cross-reactivity in polyclonal IgG1 responses against SARS-CoV-2 variants of concern
AU - van Rijswijck, Danique M. H.
AU - Bondt, Albert
AU - Hoek, Max
AU - van der Straten, Karlijn
AU - Caniels, Tom G.
AU - Poniman, Meliawati
AU - Eggink, Dirk
AU - Reusken, Chantal
AU - de Bree, Godelieve J.
AU - Sanders, Rogier W.
AU - van Gils, Marit J.
AU - Heck, Albert J. R.
N1 - Funding Information: This research received funding through the Dutch Research Council (NWO) funding the Netherlands Proteomics Centre through the X-omics Road Map program (project 184.034.019) and Gravitation Subgrant 00022 from the Institute for Chemical Immunology (D.M.H.v.R. and A.B.). A.J.R.H. acknowledges support from the Netherlands Organization for Scientific Research (NWO) through the Spinoza Award SPI.2017.028 to A.J.R.H., R.W.S. acknowledges support from the Netherlands Organization for Scientific Research (NWO) through a Vici grant 91818627. The authors also acknowledge support from the Bill & Melinda Gates Foundation through grant INV-024617 (M.J.v.G.). M.J.v.G. is a recipient of an Amsterdam UMC AMC Fellowship. Publisher Copyright: © 2022, The Author(s).
PY - 2022/12/1
Y1 - 2022/12/1
N2 - Existing assays to measure antibody cross-reactivity against different SARS-CoV-2 spike (S) protein variants lack the discriminatory power to provide insights at the level of individual clones. Using a mass spectrometry-based approach we are able to monitor individual donors’ IgG1 clonal responses following a SARS-CoV-2 infection. We monitor the plasma clonal IgG1 profiles of 8 donors who had experienced an infection by either the wild type Wuhan Hu-1 virus or one of 3 VOCs (Alpha, Beta and Gamma). In these donors we chart the full plasma IgG1 repertoires as well as the IgG1 repertoires targeting the SARS-CoV-2 spike protein trimer VOC antigens. The plasma of each donor contains numerous anti-spike IgG1 antibodies, accounting for <0.1% up to almost 10% of all IgG1s. Some of these antibodies are VOC-specific whereas others do recognize multiple or even all VOCs. We show that in these polyclonal responses, each clone exhibits a distinct cross-reactivity and also distinct virus neutralization capacity. These observations support the need for a more personalized look at the antibody clonal responses to infectious diseases.
AB - Existing assays to measure antibody cross-reactivity against different SARS-CoV-2 spike (S) protein variants lack the discriminatory power to provide insights at the level of individual clones. Using a mass spectrometry-based approach we are able to monitor individual donors’ IgG1 clonal responses following a SARS-CoV-2 infection. We monitor the plasma clonal IgG1 profiles of 8 donors who had experienced an infection by either the wild type Wuhan Hu-1 virus or one of 3 VOCs (Alpha, Beta and Gamma). In these donors we chart the full plasma IgG1 repertoires as well as the IgG1 repertoires targeting the SARS-CoV-2 spike protein trimer VOC antigens. The plasma of each donor contains numerous anti-spike IgG1 antibodies, accounting for <0.1% up to almost 10% of all IgG1s. Some of these antibodies are VOC-specific whereas others do recognize multiple or even all VOCs. We show that in these polyclonal responses, each clone exhibits a distinct cross-reactivity and also distinct virus neutralization capacity. These observations support the need for a more personalized look at the antibody clonal responses to infectious diseases.
UR - http://www.scopus.com/inward/record.url?scp=85139886292&partnerID=8YFLogxK
U2 - https://doi.org/10.1038/s41467-022-33899-1
DO - https://doi.org/10.1038/s41467-022-33899-1
M3 - Article
C2 - 36243713
SN - 2041-1723
VL - 13
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 6103
ER -