TY - JOUR
T1 - The transcription factor GATA3 is essential for the function of human type 2 innate lymphoid cells
AU - Mjösberg, Jenny
AU - Bernink, Jochem
AU - Golebski, Korneliusz
AU - Karrich, Julien J.
AU - Peters, Charlotte P.
AU - Blom, Bianca
AU - te Velde, Anje A.
AU - Fokkens, Wytske J.
AU - van Drunen, Cornelis M.
AU - Spits, Hergen
PY - 2012
Y1 - 2012
N2 - Type 2 innate lymphoid cells (ILC2s) are part of a large family of ILCs that are important effectors in innate immunity, lymphoid organogenesis, and tissue remodeling. ILC2s mediate parasite expulsion but also contribute to airway inflammation, emphasizing the functional similarity between these cells and Th2 cells. Consistent with this, we report that the transcription factor GATA3 was highly expressed by human ILC2s. CRTH2(+) ILC2s were enriched in nasal polyps of patients with chronic rhinosinusitis, a typical type 2-mediated disease. Nasal polyp epithelial cells expressed TSLP, which enhanced STAT5 activation, GATA3 expression, and type 2 cytokine production in ILC2s. Ectopic expression of GATA3 in Lin(-)CD127(+)CRTH2(-) cells resulted in induction of CRTH2 and the capacity to produce high amounts of type 2 cytokines in response to TSLP plus IL-33. Hence, we identify GATA3, potently regulated by TSLP, as an essential transcription factor for the function of human ILC2s
AB - Type 2 innate lymphoid cells (ILC2s) are part of a large family of ILCs that are important effectors in innate immunity, lymphoid organogenesis, and tissue remodeling. ILC2s mediate parasite expulsion but also contribute to airway inflammation, emphasizing the functional similarity between these cells and Th2 cells. Consistent with this, we report that the transcription factor GATA3 was highly expressed by human ILC2s. CRTH2(+) ILC2s were enriched in nasal polyps of patients with chronic rhinosinusitis, a typical type 2-mediated disease. Nasal polyp epithelial cells expressed TSLP, which enhanced STAT5 activation, GATA3 expression, and type 2 cytokine production in ILC2s. Ectopic expression of GATA3 in Lin(-)CD127(+)CRTH2(-) cells resulted in induction of CRTH2 and the capacity to produce high amounts of type 2 cytokines in response to TSLP plus IL-33. Hence, we identify GATA3, potently regulated by TSLP, as an essential transcription factor for the function of human ILC2s
U2 - https://doi.org/10.1016/j.immuni.2012.08.015
DO - https://doi.org/10.1016/j.immuni.2012.08.015
M3 - Article
C2 - 23063330
SN - 1074-7613
VL - 37
SP - 649
EP - 659
JO - Immunity
JF - Immunity
IS - 4
ER -